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Representative images of immunohistochemical results. Expression of calreticulin, prohibitin and HSP60 <t>in</t> <t>adrenocortical</t> carcinomas <t>(ACC),</t> adrenocortical adenomas (ACA) and normal tissues (N) ( A ). Calreticulin and prohibitin but not HSP60 were overexpressed in ACC compared with adrenocortical adenomas (ACA) and normal tissues ( B ).
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Representative images of immunohistochemical results. Expression of calreticulin, prohibitin and HSP60 in adrenocortical carcinomas (ACC), adrenocortical adenomas (ACA) and normal tissues (N) ( A ). Calreticulin and prohibitin but not HSP60 were overexpressed in ACC compared with adrenocortical adenomas (ACA) and normal tissues ( B ).

Journal: Diagnostic Pathology

Article Title: A comparative proteomic study identified calreticulin and prohibitin up-regulated in adrenocortical carcinomas

doi: 10.1186/1746-1596-8-58

Figure Lengend Snippet: Representative images of immunohistochemical results. Expression of calreticulin, prohibitin and HSP60 in adrenocortical carcinomas (ACC), adrenocortical adenomas (ACA) and normal tissues (N) ( A ). Calreticulin and prohibitin but not HSP60 were overexpressed in ACC compared with adrenocortical adenomas (ACA) and normal tissues ( B ).

Article Snippet: Frozen ACC and normal adjacent adrenocortical tissues were first homogenized using a sample grinding kit (GE Healthcare) with a lysis buffer (7 M urea, thiourea 2 M, 2% CHAPS, 18 mM DTT, 1 mM PMSF, 0.5% IPG buffer pH3~10NL), and then the extracts were centrifuged at 12,000 g, 4°C, for 1 hr.

Techniques: Immunohistochemical staining, Expressing